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辽宁新病害核桃枝枯病病原鉴定

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[目的]为了对辽宁省近年发生的新病害—核桃枝枯病的病原进行鉴定,[方法]采集辽宁省葫芦岛市建昌县核桃枝枯病样品,通过对病原菌形态观察、致病性测定,以及rDNA-ITS序列分析.[结果]经分离纯化获得病原菌,通过柯赫氏法则证病,接种后出现的病害症状与直接采集到的病害标样症状一致,确定其为致病菌;对病原菌进行传统的形态学观察,病原菌分生孢子器和分生孢子的形态与胡桃楸拟茎点霉较相似;将待测菌株rDNA-ITS序列与GenBank中相关菌株的ITS序列进行同源性比较,与Phomopsis sp.(GenBank收录号:HM595508.1)的同源性达到99%.[结论]确定辽宁地区新病害核桃枝枯病的病原菌为胡桃楸拟茎点霉Phonopsis juglandina.
Identification of Walnut Twig Blight in Liaoning Province
[Objcetive]The walnut twig blight is a new disease in Liaoning province.The objective of the study was to identify the pathogen.[Method] The walnut twig blight standard samples were collected from Jianchang County,Huludao City,Liaoning Province.The pathogen was identified by morphology,pathogenicity and ribosomal DNA-ITS.[Results] The isolated fungus was used for department's law of disease and syndrome.Disease symptoms after inoculation were the same as that on the collected samples,which showed the fungus was the pathogen.The pycnidia and conidia' morphology of the pathogen were similar to that of Phomopsis juglandina.Comparing the measured sequence with the related sequence in GenBank,the homology was 99%.[Conclusion] The studies suggested that the pathogen causing the walnut twig blight in Liaoning Province was Phomopsis juglandina.

WalnutWalnut twig blightPhomopsis juglandina

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辽宁省经济林研究所,辽宁大连116031

核桃 枝枯病 拟茎点霉

2011207003

2013

果树学报
中国农业科学院郑州果树研究所

果树学报

CSTPCDCSCD北大核心
影响因子:1.486
ISSN:1009-9980
年,卷(期):2013.30(4)
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