Prediction and analysis of the signal peptide and secreted proteins in soft rot bacteria XJ8-3-3 genome ORFs of banana
[Objective]According to the whole-genome sequencing results of soft rot bacteria XJ8-3-3 in banana,Prediction and analysis of signal peptide for this bacteria can help us to research secreted proteins,which benefits confirming the molecular mechanisms of the interaction between banana and pathogenic microorganisms.[Method] Using several software of SignalPv4.1,TMHMMv2.0,THUMBUP,TargetP1.1,LipoP1.0,TatP1.0 and big-PI predictor,4 313 ORFs of the genome of banana soft rot bacteria were predicted and analyzed.In addition,the function of ORFs where were the signal peptides was predicted by Swissprot database.[Result] We found that there were 218 ORFs containing signal peptide accounting for 5.05％ of the ORFs coded by genome,in which there were 184 ORFs belonging to type Ⅰ,32 ORFs belonging to type Ⅱ,2ORFs belonging to type CYT,but Prepilin-like type was not discovered.Signal peptides had 22.56 amino acids evenly,and most of all were nonpolar.In all secreted proteins,122 had divinable function,and others were function-unknown putative protein.[Conclusion]The analysis of the signal peptide and secreted proteins in banana soft rot bacteria XJ8-3-3 genome ORF revealed a lot of pathogenic factors such as pectinase,protease,cellase and some assumed pathogenic factors wich could act as candidate genes for the further studies of the interaction between banana and pathogenic bacteria
Banana soft rot bacteriaSignal peptideSecreted protein
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