Validation of the generality of molecular markers for seedless fruit in grape
[Objective]The natural population of grape(Vitis vinifera L.)was used to evaluate the uni-versality of 9 molecular markers for seedless fruit in order to provide technical support for the breeding of new seedless grape varieties.[Methods]DNA was extracted from healthy and young samples of a natural population consisting of 88 seedless germplasmes and 120 nucleated germplasmes.PCR amplifi-cation was performed using 9 reported molecular markers for seedless fruit of grape.Then,the PCR products were detected by 1.5％agarose gel electrophoresis and capillary electrophoresis,and the spe-cific bands were analyzed.The accuracy rate and seedless detection rate were calculated respectively to verify the versatility of 9 molecular markers for seedless fruit of grape.[Results]Among 208 grape germplasmes,16 germplasmes were detected by SCAR marker GSLP1-569,including 14 seedless germplasmes and 2 seeded germplasmes.The 14 seedless varieties were Summer Black,Changwuhebai Etc.among others.And,among them,12 germplasmes were Thompson Seedless and its derivatives.The identification accuracy and nuclear-free detection rate were 57.12％and 90.51％,respectively.Addi-tionally,the 1080 bp specific band was amplified by SCC8-1080 in 53 seedless germplasmes and 19 seeded germplasmes,and the statistical identification accuracy and seedless detection rate were 72.20％and 79.18％,respectively.Moreover,the 2000 bp specific band was amplified by SCF27-2000 in 87 seedless germplasmes and 55 seeded germplasmes.The statistical identification accuracy and seedless detection rate were 75.38％and 67.82％,respectively.Furthermore,a total of 6 isotopic point and 8 gen-otypes were detected by the SSR marker p1-VvAGL11.The chi-square test showed that the allele 250 bp was significantly correlated with the nuclear-free phenotype and 257 bp was significantly correlated with nucleated phenotype.The genotype 250/250 was significantly correlated with the nuclear-free phe-notype,and the genotype 257/257 was significantly correlated with the nuclear phenotype.The statisti-cal identification accuracy and nuclear-free detection rate of the marker were 88.47％and 87.74％,re-spectively.A total of 3 isotopic point and 5 genotypes were detected by the marker p2-VvAGL11.The chi-square test showed that the allele 171 bp was significantly correlated with the nuclear-free pheno-type.The 158 bp was significantly correlated with the nuclear phenotype.The genotype 158/171 was significantly correlated with the seedless phenotype,and the genotype 158/158 was significantly corre-lated with the nuclear phenotype.The accuracy of marker identification and seedless detection rate were 67.43％and 77.22％,respectively.A total of 14 isotopic point and 30 genotypes were detected by the marker p3-VvAGL11.The chi-square test showed that the allele 195 bp was significantly correlated with the nuclear-free phenotype and 185 bp was significantly correlated with the nucleated phenotype.The genotype 185/195 was significantly associated with the nuclear-free phenotype.The genotype 185/185 was significantly correlated with the nuclear phenotype,and the accuracy and nuclear-free detection rate of the marker were 71.94％and 90.72％,respectively.A total of 24 isotopic point and 75 genotypes were detected by the marker 5U_VviAGL11.The chi-square test indicated that the allele 315 bp was significantly correlated with the nuclear-free phenotype and 305 bp was significantly associated with nu-cleated phenotype.The genotype 307/315 was significantly correlated with the nuclear-free phenotype.The accuracy and nuclear-free detection rate of the marker were 68.47％and 90.80％,respectively.A to-tal of 8 isotopic point were detected in the marker VMC7F2,and the allele 197 bp was significantly cor-related with the seedless phenotype by chi-square test.199 bp was significantly correlated with one phe-notype.The genotype 197/199 was significantly associated with the nuclear-free phenotype.The geno-type 199/199 was significantly correlated with the nuclear phenotype,and the accuracy and non-nuclear detection rate of the marker were 67.99％and 79.10％,respectively.A total of 9 isotopic point and 21 genotypes were detected by the marker VVSD10.The chi-square test showed that the allele 105 bp was significantly correlated with the nuclear-free phenotype,and the genotype 105/105 was significantly correlated with the nuclear-free phenotype.The identification accuracy and nuclear-free detection rate of the marker were 61.60％and 63.03％,respectively.[Conclusion]Among the SCAR type markers,SCF27-2000 had the highest accuracy and true positive rate,and the performance was the best.And,GSLP1-569 was more suitable for the hybrid offspring of Thompson seedless series.Among the SSR markers,p1-VvAGL11 had good accuracy and seedless detection rate,and the false negative and false positive were low,showing the best performance,while p3-VvAGL11 and 5U_VviAGL11 had higher seedless detection rate,and 5U_VviAGL1 1 contained more genetic information.
国家科技期刊平台
NSTL
万方数据
