PNV@LIR纳米囊泡的制备及其细胞功能验证
PNV@LIR preparation of nanovesicles and validation of their cellular functions
陈奇英 1黄清昱 1孙晟甲 1吴帮卫 1阿力木江·买买提江1
作者信息
- 1. 复旦大学附属华山医院心内科,上海 200040
- 折叠
摘要
利用胞吞法制备尺寸均匀的血小板包裹利拉鲁肽的纳米囊泡.从全血中经过离心、重悬和洗涤得到大小均匀的血小板纳米囊泡,进一步将利拉鲁肽与血小板纳米囊泡37 ℃恒温摇床共孵育4 h,得到血小板载利拉鲁肽纳米囊泡(PNV@LIR).检测其理化和功能指标:PNV@LIR纳米囊泡的颗粒大小、微观形貌、Zeta电位、表面蛋白受体、细胞毒性和抗氧化损伤能力.结果显示,制备得到的PNV@LIR为稳定的140 nm的囊泡状,PNV@LIR的Zeta电位值为(-23.2±1.85)mV.SDS-PAGE分析证实了 PNV@L1R表面的膜蛋白结构的存在.体外细胞实验显示:PNV@LIR纳米囊泡生物相容性良好,且具有优异的抗氧化损伤能力.研究应用离心和共孵育相结合的方法成功制备出大小均一的PNV@LIR纳米囊泡,SDS-PAGE结果表明其表面保留了血小板本身的蛋白受体;体外细胞实验表明PNV@LIR纳米囊泡无细胞毒性,且具有明显的抗氧化应激损伤能力.
Abstract
The purpose of this article is to prepare platelet coated liraglutide nanovesicles with uniform size by endocytosis.Platelet nanovesicles of uniform size were obtained from whole blood by centrifugation,re-suspension and washing.Liraglutide was further incubated with platelet nanovesicles for 4 h at 37 ℃ sha-king table.Then platelet-loaded liraglutide nanocapsules(PNV@LIR)were obtained.The physicochemical and functional indexes of the nanoparticles were detected including the particle size,microstructure,Zeta potential,surface protein receptors,cytotoxicity and antioxidant damage ability of PNV@LIR.The results show that the obtained PNV@LIR was a stable 140 nm vesicle shape,and the Zeta potential value of PNV@LIR was(-23.2±1.85)mV.SDS-PAGE was used to verify the existence of membrane protein structure on the surface of PNV@LIR.In vitro cell experiments showed that PNV@LIR nanovesicles had good biocompatibility and excellent anti-oxidative damage ability.Therefore,homogeneous PNV@LIR nanoveses were successfully prepared by a combination of centrifugation and co-incubation.The surface of the nanoveses retained the platelet protein receptors.In vitro cell experiments showed that PNV@LIR vesicles were non-cytotoxic and had obvious anti-oxidative damage ability.
关键词
共孵育/血小板/利拉鲁肽Key words
Co-incubation/Platelet nanovesicles/Liraglutide引用本文复制引用
出版年
2024
NETL
NSTL
万方数据