Effect of allicin combined with hyperthermia on radiosensitization of human lung cancer H1299 cells
It was investigated the effect of allicin combined with hyperthermia on radiosensitization of human lung cancer H1299 cells.Human lung cancer H1299 cells were culturedin vitro and divided into five groups:control group,allicin treatment group,hyperthermia group,chemotherapy group and allicin + hyperthermia + chemotherapy group.The cell survival rate was detected by MTT assay.Clonal formation assay was used to detect cell proliferation.The cell cycle,apoptosis and mitochondrial membrane potential(ΔΨm)were detected by flow cytometry.The expression levels of PCNA,Bax and Bcl-2 genes were detected by qRT-PCR and Western blot.The results showed that compared with the control group,allicin had a dose-dependent inhibitory effect on H1299 cell growth,and the optimal quality concentration for 48 h was 20 µg/mL.After treatment with 20 µg/mL allicin,45℃heating and 8 Gy X-ray irradiation,cell viability and clone formation rate were decreased.The results showed that all three treatments could reduce cell viability and clone formation rate.The cell cycle was blocked in G1 phase,the apoptosis rate in early stage was(4.75±0.28)%(P＜0.01),the apoptosis rate in late stage was(19.30±0.49)%(P＜0.01),ΔΨm decreased to 3.71±0.50(P＜0.01),and the expression of proliferation marker gene PCNA decreased.Apoptosis-related Bax gene expression increased,Bcl-2 gene expression decreased(P＜0.01).Allicin combined with hyperthermia can increase the radiosensitization of human lung cancer H1299 cells and induce apoptosis by up-regulating Bax and down-regulating Bcl-2.
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