胃癌中circRNA相关ceRNA预后调控网络的生成方法
Generation method of prognostic regulatory networks of ceRNA related with circRNA in gastric cancer
杜晓昕 1于欢 1王波 1张剑飞 1周薇1
作者信息
- 1. 齐齐哈尔大学 计算机与控制工程学院,黑龙江 齐齐哈尔 161006
- 折叠
摘要
为描述胃癌中circRNA相关的竞争内源性RNA(ceRNA)调控网络,从GEO和TCGA数据库中检索胃癌circRNA,miRNA,mRNA表达谱,识别差异表达的RNA,利用加权基因共表达网络(WGCNA)鉴定关键模块.对关键模块基因做Lasso-Cox回归分析,同时进行生存分析.差异表达分析鉴定了115个circRNA和628个miRNA,WGCNA构建15个circRNA模块和11个mRNA模块.Lasso-Cox分析筛选出4个mRNA.筛选了一系列失调的circRNA,miRNA和mRNA,构建了胃癌中circRNA相关的ceRNA网络.构建的网络有助于加深对circRNA相关调控机制的理解.
Abstract
To describe the regulatory networks of competing endogenous RNA(ceRNA)related with circRNA in gastric cancer,the expression profiles of gastric cancer circRNA,miRNA and mRNA were retrieved from GEO and TCGA databases,and differentially expressed RNAs were identified,key modules were identified using the weighted gene co expression network(WGCNA).Lasso-Cox regression analysis on key module genes was performed,while survival analysis was conduct.Differential expression analysis identified 115 circRNAs and 628 miRNAs,WGCNA constructed 15 circRNA modules and 11 mRNA modules.Lasso Cox analysis identified 4 mRNAs.A series of dysregulated circRNAs,miRNAs,and mRNAs were screened,and a ceRNA network related to circRNA in gastric cancer was constructed.The constructed network can deepen the understanding of circRNA related regulatory mechanisms.
关键词
胃癌/WGCNA/Lasso-Cox回归分析/ceRNA调控网络Key words
gastric cancer/WGCNA/Lasso-Cox regression analysis/ceRNA regulatory network引用本文复制引用
基金项目
黑龙江省高等学校自然科学类青年创新人才项目(145209206)
出版年
2024
国家科技期刊平台
NSTL
万方数据