Isolation and purification of purslane polysaccharides and inhibitory effects on the α-amylase
Using dried and fresh purslane as raw materials to extract,remove protein,decolorizeand purify by column chromatography to obtain dried purslane polysaccharides(POP-Dc1,POP-Dc2)and fresh purslane polysaccharides(POP-Fc1,POP-Fc2).The inhibitory effects of two purslane polysaccharides on α-amylase activity were determined using the colourimetric method of 3,5-dinitrosalicylic acid(DNS).The results showed that the isolated and purified purslane polysaccharides exhibited certain inhibitory effects on α-amylase activity and the inhibitory ability was in the following magnitude:POP-Fc1>POP-Dc1>POP-Fc2>POP-Dc2,among which the strongest inhibitory effect was observed for POP-Fc1,with an inhibitory rate of (58.2±0.2)％ at 2.0 mg/mL.So that,separated and purified polysaccharides from fresh purslane can better inhibit α-amylase activity,which is expected to be used in the development of hypoglycemic drugs and health food.
purslane polysaccharidesseparation and purificationα-amylase
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