Synthesis and spectral properties of phenanthro[9,10-d]imidazole-based fluorescence probe towards hypochlorous acid
The method of fluorescence probe,due to its advantages of high sensitivity,strong selectivity,and real-time response,has been widely applied in the detection of organisms and environments.Therefore,the development of structurally diverse fluorescence probes is of great theoretical and practical significance.Based on phenanthro[9,10-d]imidazole luminophore,a reactive fluorescent probe(PIP-BH)towards ClO-which contains a benzoyl hydrazine moiety was designed and synthesized,and its structure was fully characterized and confirmed.The aggregation-caused quenching(ACQ)effect of the probe PIP-BH in aqueous phase was investigated by UV and fluorescence spectra,further confirmed through the Tyndall effect and scanning electron microscopy(SEM)tests.In the MeOH/PBS(fw=40％,PBS 0.01 mol·L-1,pH=7.4)buffer solution,the fluorescence response characteristics of the probe PIP-BH towards ClO-were discussed in detail by the fluorescence spectrum.The probe PIP-BH can selectively recognize ClO-in the range of wide pH values(5.0～1 1.0),accompanied by the blue shift of emission wavelength,which demonstrates that PIP-BH has good application value in real detection.
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