Protective effect of metformin on liver tissue of D-galactose-induced aging model mice
The purpose is to investigate the impact of metformin on liver tissue in aging mice.After induction gavage,the mice were euthanized,and their body quality and liver quality were measured.HE staining was utilized to observe morphological changes in the liver tissue.Additionally,activity of reactive oxygen species(ROS),total antioxidant capacity(T-AOC),catalase(CAT),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and the concentrations of reduced glutathione(GSH),malondialdehyde(MDA)in mouse liver tissue.Simultaneously,the expression levels of genes related to aging secretion phenotype including P53,P21,P1 6,LaminB1,HMGB1,IL-6,TNF-α,CXCL-10 and Rad51 were also detected.The results revealed that compared with the control group(Ctrl),the ROS,CAT activity,and MDA concentration in the induced aging group(IA)were significantly increased,while the T-AOC,SOD,GSP-Px activity and GSH content were significantly reduced.Additionally,the mRNA expression levels of genes related to aging secretion phenotype were all elevated.Compared with the IA group,in the induced aging+metformin group(IA+Metf),ROSlevels,CAT activity,and MDA concentration were significantly reduced,while the activities of T-AOC,SOD,GSP-Px and GSH contents increased.Further more,the mRNA expression levels of genes related to aging secretion phenotypein mouse liver are reduced.In summary,metformin has an improving effect on liver damage in D-galactose-induced aging model mice,which can provide experimental evidence for the utilization of metformin in anti-aging.