Effect of liquid pig manure microbial fertilizer on soil microbial community of rice
Microbial fertilizers are increasingly popular as emerging fertilizers.To investigate the effects of microbial fertilizers on soil microbial communities,rice pot planting experiments were conducted on black and saline-alkali soil,during which rice was irrigated with pig wastewater microbial fertilizers and the test soils were collected for 16S rRNA high-throughput sequencing.The results showed that the application of pig wastewater microbial fertilizer increased the Chao and Shannon index of the microbial community in black soil,but decreased the Chao and Shannon index of the microbial community in salt-alkaline soil.Based on principal component analysis and P test,it was observed that there was a significant effect of microbial community among different groups.The Veen plots showed that the application of fertilizer increased the number of OTUs unique to the bacterial and fungal communities in black soil,but the opposite phenomenon was observed in the salt-alkaline soil samples.In soil community abundance analysis,found that the relative abundance of microbial communities beneficial to plant growth was enhanced in black and saline-alkali soil,especially among the microorganisms of the Bacillaceae and Rhizobiaceae.In addition,set up different microbial fertilizer concentration treatment groups and rice planting experiments in different soils,which can provide a scientific and efficient fertilizer application basis for local rice planting.
black soilhigh-throughput sequencingmicrobial fertilizerricesaline-alkali soil