Identification of Apple Leaf Spot Pathogen and Screening of Fungicides
To identify the pathogen causing leaf spot of Malus pumila,samples of Malus pumila leaf spot were collected,and the isolated strains were isolated and identified using tissue separation method,morphology and molecular biology.The pathogenicity of the isolated strains was determined by mycelium plugs attachment method.The results showed that the pathogen causing apple leaf spot disease in this area was Alternaria alternata,which provided a reference for the prevention and control of leaf spot disease.Four kinds of chemical agents had inhibitory effects on pathogens.the results found that the EC50 of 45%prochloraz EW(EC50=0.521 5 mg·L)was the smallest and 75%chlorothalonil WP(EC50=1 080.19 mg·L)was the largest among the four agents.The highest inhibition rate of the four agents was 45%prochloraz EW,and the lowest inhibition rate was 75%chlorothalonil WP,when diluted 2 000 times,the bacteriostatic rates were 82.67%and 58.26%,respectively.So 45%prochloraz EW could be preferentially selected for the prevention and control of apple leaf spot caused by Alternaria alternata.
pathogen of apple leaf spotAlternariaidentification of pathogenscreening of fungicides
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