Identification and Analysis of Gasterophilus spp.Larvae of Equus ferus in Gansu Anxi Extreme-arid Desert National Nature Reserve:Evidence from Morphological and Mitochondrial COI Gene
Gasterophilus spp.is a common and important parasite of the family Equidae.At present,nine species of Gasterophilus spp.have been reported in the world.The geographical distribution of these different species obviously has various characteristics,showing apparent differences in the species composition structure of Gasterophilus infection in different regions.In order to explore the species composition of Equus ferus infected with Gasterophilus larvae in Gansu Anxi Extra-arid National Nature Reserve,the Equus ferus were dewormed and maintained in the reserve in 2021,and the dead individuals were dissected to collect Gasterophilus larvae.A total of 875 Gasterophilus larvae were collected.Firstly,the morphological observation and identification of the third instar larvae were applied.According to the third instar larva,the shape of each body,the spine page smooth shape,pseudo head small thorn clear structure,the third instar larvae were considered as Gasterophilus pecorum;Secondly,for the first and second instar larvae that were difficult to identify based on morphological characters,the mitochondrial cytochrome oxidase subunit(COI)gene was amplified for molecular identification.Above all,the larvae were all identified to be G.pecorum based on both morphological and molecular evidence.
Equus ferusGasterophilus sppmitochondrial COI genemolecular identification
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