Biological Features of Two Types of Olea europaea Leaf Spot Disease
Using two common Olea europaea diseases,typically Pseudocercospora cladosporioides and Phomopsis olea-europaea that normally occured on Wudu District of Longnan City in Gansu Province as test pathogens,combined with a constant temperature method to cultivate fungal mycelia,eventually analysed its effects on the mycelial growth and spore production under following treating conditions:like five culture mediums,six carbon sources,six nitrogen sources,six carbon-nitrogen ratios,five pH values,and six temperatures,especially aiming at providing reference datas for the isolation and purification of other O.europaea pathogenic fungi.The results showed that the two test pathogenic fungi performed best in PDA culture medium,with soluble starch being the best carbon source,tryptone being the best nitrogen source,the optimal carbon-nitrogen ratio being 30:1,the optimal pH value being 7,and the optimal cultivation temperature being 20～25 ℃,respectively.
Olea europaeapathogenic fungiculture mediumsspore production
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