摘要
以甘肃省陇南市武都区2种普遍发生的油橄榄叶部病害(油橄榄灰斑叶霉病、油橄榄叶枯病)为供试病菌,采用恒温培养真菌菌丝的方法,研究5种培养基、6种碳源、6种氮源、6种碳氮比、5个pH值、6个温度等分别对2种病菌菌丝生长和分生孢子的影响,以期为其他油橄榄病害病原真菌的分离纯化提供参考依据.结果表明:2种供试病原真菌在PDA培养基中表现最好,可溶性淀粉为最佳碳源,蛋白胨为最佳氮源,最佳碳氮比为30:1,最佳pH值为7,最佳培养温度为20～25 ℃.
Abstract
Using two common Olea europaea diseases,typically Pseudocercospora cladosporioides and Phomopsis olea-europaea that normally occured on Wudu District of Longnan City in Gansu Province as test pathogens,combined with a constant temperature method to cultivate fungal mycelia,eventually analysed its effects on the mycelial growth and spore production under following treating conditions:like five culture mediums,six carbon sources,six nitrogen sources,six carbon-nitrogen ratios,five pH values,and six temperatures,especially aiming at providing reference datas for the isolation and purification of other O.europaea pathogenic fungi.The results showed that the two test pathogenic fungi performed best in PDA culture medium,with soluble starch being the best carbon source,tryptone being the best nitrogen source,the optimal carbon-nitrogen ratio being 30:1,the optimal pH value being 7,and the optimal cultivation temperature being 20～25 ℃,respectively.
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