Content Determination of Trigonelline in Wild and Cultivated Himalayan Mirabilis by HPLC
Objective: To set up a method for determining the content of trigonelline in Himalayan Mirabilis by HPLC, and compare the differences of the content of trigonelline between wild and cultivated Himalayan mirabilis. Methods: The ion pair chromatography was used, chromatographic column: Elite Sinopak C18 (250 mm× 4.6 mm, 5 μm), mobile phase: methanol-glacial acetic acid-0.05% sodium dodecyl sulfate (20:0.1:80), the flow velocity: 1.2 mL/min, detection wavelength: 265nm, column temperature: 30℃ . Results: Trigonelline showed good linear relationship in the range from 0. 2473 to 1.2360 μg/mL and peak area, r=0.9997. The average recovery rate was 99.21%, RSD=0.50% (n=6). The contents of trigonelline in cultivated and wild Himalayan mirabilis were 0.5876 and 0.3226 mg/g. Conclusion: The contents of trigonelline contained in cultivated Himalayan mirabilis are higher than these contained in wild Himalayan mirabilis, which could provide theoretical evidence for artificial plant, popularization and application of Himalayan mirabilis.
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万方数据
维普
