Effects of Catalpol on Myocardial Fibrosis and Myocardial Cells Morphology in Rats with Type 2 Diabetes Mellitus
Objective:To study the effects of catalpol on myocardial fibrosis and morphology in the rats with type 2 diabetes mellitus(T2DM).Methods:SPF grade healthy male Wistar rats were randomized into 10 ones in the normal group and 80 ones in the experiment group using random number table method.The rats in the experimental group accepted peritoneal injection of STZ solution,15 mg/kg,to prepare T2DM models after feeding with high-fat and high-sugar fodder for eight weeks.Rat models were divided into the model group,melbine(DMBG)group,low,moderate and high dose groups of catalpol according to FPG and body mass.DMBG group was drenched with DMBG suspension,90 mg/kg,low,moderate and high dose groups of catalpol accepted intragastric administration of catalopl at 2.5,5 and 10 mg/kg respectively,the normal group and the model group accepted equivalent volume of physiological saline by gavage,once each day,drenched for 12 weeks.Since the last dose,to detect FPG,immunohistochemical method was applied to detect the expressions of TGF-β1 in myocardial tissue;HE staining to observe the morphology of myocardial cells.Results:Compared with the model group,the levels of FPG and myocardial TGF-β1 reduced in high,moderate and low dose groups of catalpol(P<0.05);there was no significant difference in myocardial fiber,cytoplasm,collagen fibers and nucleus when high dose group was compared with the normal group.The myocardial fibers were disordered or irregular,partly dissolved,broken,even distributed in small flakes,the cytoplasm was stained unevenly,with moderate or slight swelling.Some nuclei were deformed,showing pyknosis,even disintegrated in the model group and low dose group.The myocardial gap widened significantly,large or small numbers of inflammatory cells or fibroblasts could be seen.the arrangement of myocardial fibers was slightly regular,and the nucleus of myocardial cells was rarely deformed in DMBG group and moderate dose group;myocardial gap is slightly widened,with fewer inflammatory cells.Conclusion:Catalpol could reduce the levels of FPG and myocardial fibrosis in T2DM rats,and it may be an effective way of protecting myocardial cells in T2DM rats.
type 2 diabetes mellitusmyocardial fibrosismyocardial cells morphologycatalpol
NETL
NSTL
万方数据
维普
