Influence of Coix Seed Oil Jointed with 5-FU on the Proliferation and Apoptosis of Colon Cancer HCT-116 Cell Line
Objective:To analyze the effects of coix seed oil and 5-FU on the proliferation and apoptosis of colon cancer HCT-116 cell line,and its mechanism.Methods:The inhibitory effects of coix seed oil for injection with different concentrations,5-FU and coix seed oil+5-FU on the growth of human colon cancer HCT-116 cell line were detected by using MTT method;cellular apoptosis conditions were measured through using flow cytometry;the expression of Survivin was checked by adopting RT-PCR.Results:Coix seed oil for injection,5-FU and coix seed oil for injection+5-FU produced certain inhibitory effects on the proliferation of human colon cancer HCT-116,the higher the concentration of coix seed oil for injection was,the longer the action time was,the higher cellular proliferation inhibitory rate was,the difference had statistical meaning between three groups(P<0.05);compared with coix seed oil for injection group and 5-FU group,cellular proliferation inhibitory rate was higher in the group of coix seed oil for injection+5-FU,and the difference showed statistical meaning(P<0.05).Cellular apoptosis rates of coix seed oil for injection group,5-FU group and the group of coix seed oil for injection+5-FU were higher than that of blank control group,and the difference was statistically significant(P<0.05);the higher the concentration of coix seed oil for injection was,the higher the apoptosis rate was,the difference had statistical meaning(P<0.05);cellular apoptosis rate in the group of coix seed oil for injection+5-FU was higher than that of 5-FU group and coix seed oil for injection group,and the difference had statistical meaning(P<0.05).the compared with blank control group,expressions Survivin mRNA lowered in coix seed oil for injection group,and the difference had statistical meaning(P<0.05);the expressions of Survivin mRNA in 5-FU group rose,and the difference showed statistical meaning(P<0.05);the expressions of Survivin mRNA in 5-FU+coix seed oil for injection group were lower than these of 5-FU group,and the difference was statistically significant(P<0.05).Conclusion:Coix seed oil for injection could inhibit the proliferation of colon cancer HCT-116 cells,induce cellular apoptosis,its combination with 5-FU could develop synergistic effects,and its mechanism might be related to the downregulated expressions of Survivin mRNA.
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