Effects of Electroacupuncture on Myocardial Injury and Mitochondrial Function in Bupivacaine Poisoned Rats
Objective:To observe the influence of electroacupuncture pretreatment on bupivacaine(BPV)-induced injury of cardiac muscle and mitochondrial function,and to discuss the mechanism of electroacupuncture at Neiguan(PC6)point against myocardial injury.Methods:Thirty male SD rats were allocated to the control group[A group,intravenous injection of 0.9%physiological saline,3 mL/(kg·min)],BPV group[B group,firstly,intravenous injection of physiological saline for 30 minutes,and then intravenous dripping of 5%BPV 2 mg/(kg·min)]and electroacupuncture group(E group)according to random number table method.Myocardial injury models were established by continuous intravenous pumping BPV,E group accepted electroacupuncture at Neiguan point for 30 minutes before modeling.Phosphomolybdic acid colorimetric method was used to detect the contents of ATP in myocardial tissue of rats,DHE staining was applied to measure the relative contents of ROS in myocardial tissue,Fluo-3/AM to detect the relative fluorescence intensity of Ca2+ in rat cardiomyocytes and ELISA to detect the activity of MPTP.Results:Compared with A group,the contents of ATP and fluorescence intensity of Ca2+ were decreased in B group,and ROS accumulated(P<0.05),which suggested that the modeling of myocardial injury was successful.Compared with B group,the time of cardiac arrest was prolonged,the contents of ATP and fluorescence intensity of Ca2+ were enhanced,MPTP was raised and the relative contents of ROS were decreased(P<0.05).Conclusion:Electroacupuncture at Neiguan point could effectively improve myocardial damage induced by bupivacaine hydrochloride,ROS and Ca2+ in mitochondria are the important material foundation,and they are closely related to MPTP.
NETL
NSTL
万方数据
维普
