Objective:To discuss the influence of marein on the histomorphology of liver and pancreas,autophagy regulation in diabetic mice.Methods:Ten db/m mice were chosen as the control group,another 30 db/m mice were selected as the model group,metformin-positive medicine group and marein-administered group according to random controlled principles with ten in each group.All the groups accepted intragastric administra-tion for eight weeks continuously,HE staining was used to observe the morphology of liver and pancreas of the mice in different groups,immunohistochemical method to detect the contents of P62 in the liver of the mice,Western Blotting method to measure the expressions of autophagy-related proteins LC3Ⅱ/I,P62,ATG5 and Beclin1 in the liver of the mice.Results:The histomorphology showed that the mice of metformin-positive medicine group and marein-administered group had a clearer structure of the liver lobules,with more disorganized hepatic cords,relieved hepatocyte ballooning,normal islet morphology,having clearer borders,there was a significant improvement in the arrangement of islet cells,and a reduction in islet cell vacuolar degeneration.Liver immunohistochemistry revealed that the expressions of P62 in the model group were higher than these of the control group,the expressions of P62 were reduced clearly under the pathological state in metformin-positive medicine group and marein-administered group.The results of Western Blotting indicated that,compared with the model group,marein and metformin could noticeably increase the expressions of autophagy-related proteins LC3Ⅱ/I,ATG5 and Beclin1 in the liver of the mice(P＜0.05),decrease the expressions of P62(P＜0.05).Conclusion:Marein could reduce the histopathological damage of liver and pancreas caused by the diabetes;and enhance the expressions of liver autophagy genes in diabetic mice.
维普
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