Regulatory Effects of Qingchang Wenzhong Prescription and Fecal Microbiota Transplantation on Intestinal Mucosal Immunity in Mice with Ulcerative Colitis
Objective:To survey the regulatory effects of Qingchang Wenzhong prescription combined with fecal microbiota transplantation on intestinal mucosal immunity in mice suffering ulcerative colitis(UC).Methods:Thirty healthy SPF-grade female C57BL/6 mice were randomized into the blank group(six ones)and the intervention group(24 ones)according to the ratio of 1:4.The mice in the blank group were allowed to drink sterile water throughout the day and began to accept intragastric administration of sterile water since the eighth day,meanwhile,fresh faeces were collected to prepare faecal filtrate;UC models were established after the intervention group had freely drank 2.5%DSS solutions for seven days,and they were randomized into the model group,Qingchang Wenzhong prescription group,fecal microbiota transplantation group,the combination group(Qingchang Wenzhong prescription and fecal microbiota transplantation),intervened for seven days.To observe general conditions of the mice,to detect body mass and faecal occult blood,to record fecal character and to calculate disease activity index(DAI).The mice were sacrificed by the end of intervention,to observe histopa-thological changes by light microscope after paraffin sections of the colon were processed by HE staining;the spleen was taken out to detect the expressions of γδT cells and CD4+T cells using flow cytometry;RT-qPCR method was used to measure the expressions of TLR2 mRNA and plgR mRNA after getting out the colon,ELISA was applied to test the contents of sIgA.Results:Compared with the mice in the blank group,DSS-induced UC mice showed marked intestinal inflammation,mainly manifesting different degrees of hematochezia,body weight loss and loose stool;when DSS was withdrew,clinical symptoms began to relieve,the mice in different intervention groups presented relatively rapid recovery,DAI and histopathological scores were reduced noticeably than these of the model group(P＜0.05).The detection results of intestinal mucosal immune-related indicators found that the contents of γδT cells and CD4+T cells and the expressions of TLR2 mRNA in the colon were raised remarkably in the model group(P＜0.05 or P＜0.01),the expressions of pIgR mRNA and sIgA in the colon were lowered than these of the blank group(P＜0.05 or P＜0.01).After one week of the treatment,the expressions of γδT cells,CD4+T cells and colonic TLR2 mRNA were clearly decreased in the intervention groups(P＜0.05),the expressions of pIgR mRNA and sIgA were obviously lifted in the colon(P＜0.05 or P＜0.01);among them,the improvements of intestinal mucosal injury were the most apparent in the combination group.Conclusion:Qingchang Wenzhong prescription combined with fecal microbiota transplantation could improve intestinal inflammation in UC mice and promote the repair of intestinal mucosal injury by affecting intestinal mucosal immune response.
维普
万方数据
