Study on Regulatory Mechanism of Fetuin B-AMPK/ACC Pathway Mediated by Zicui Jiangtang Prescription on Insulin Resistance in Liver of High-fat-induced Obese Mice
Objective:To explore the mechanism of improving insulin resistance in obese C57BL/6J mice via Fetuin B-AMPK/ACC pathway medicated by Zicui Jiangtang prescription.Methods:The models with insulin resistance(IR)were established by feeding C57BL/6J mice high-fat diet,and they were randomly allocated to the model group,Zicui Jiangtang prescription group,metformin group,the combination group(Zicui Jiangtang prescription+metformin),the mice fed normal diet were chosen as blank control group.To observe the mice's body mass,liver mass,glucose tolerance,insulin resistance index(IRI),the expressions of liver Fetuin B,AMPK,ACC mRNA,liver Fetuin B,AMPKα1,ACC,P-AMPKαT183/T172 and P-ACCS79 protein.Results:Zicui Jiangtang prescrip-tion could improve the mice'glucose tolerance,reduce area under the curve,lower IRI,liver mass and hepatocrit,cut down the expressions of liver Fetuin B,ACC mRNA and Fetuin B protein,up regulate the levels of AMPK mRNA,P-AMPKαT183/T172 and P-ACCS79/ACC protein.Conclusion:Zicui Jiangtang prescription could alleviate IR of liver in obese mice possibly via Fetuin B-AMPK/ACC signaling pathway.
维普
万方数据
