Objective:To explore the mechanism of benefiting-kidney invigorating-spleen herbs Chinese yam-Duzhong(eucommiae cortex)in the treatment of osteoporosis using network pharmacology.Methods:The active ingredients and related targets of Chinese yam-Duzhong were searched by TCM database(TCMSP),and the network diagram of"active ingredients-targets"was made by using Cytoscape3.7.2.The targets related to osteoporosis were collected using GeneCards database,the intersection targets were obtained by mapping the drug targets to the disease targets,and uploaded to STRING 11.0 database to build PPI,which was imported into Cytoscape 3.7.2,processed and analyzed.The key targets were uploaded to Metascape platform to carry out GO enrichment analysis and KEGG pathway enrichment analysis.Results:The study obtained 38 active ingredients from Chinese yam and Duzhong,225 corresponding targets and 1376 targets related to the disease.There were 80 core targets of medicine-disease action,including albumin,interleukin 6(IL6),adenylate kinase isoenzyme 1,vascular endothelial growth factor(VEGF),tumor suppressor p53,tumour necrosis factor,MMP9 and others.KEGG enrichment analysis displayed that the potential pathway acting on osteoporosis mainly included AGE-RAGE signal pathways of diabetic complication,T cell receptor signal pathway,p53 signal pathway,TGF-β signal pathway and HIF-1 signal pathway.Conclusion:This study has primarily discussed the"multi-pathway,multi-target"mechanism of Chinese yam-Duzhong,the couplet medicines which could benefit the kidney and invigorate spleen,in the treatment of osteoporosis,wishing to provide the research thinking and theoretical foundation for further exploration into the treatment of osteoporosis by benefiting-kidney invigorating-spleen method.
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