Objective:To explore the mechanism of Mahuang Fuzi Xixin decoction in treating bradyarrhy-thmia based on network pharmacology.Methods:Chemical ingredients and the targets of Mahuang(ephedrae herba),Fuzi(aconiti lateralis radix praeparata)and Xixin(Asari radix et rhizoma)in Mahuang Fuzi Xixin decoction were searched from TCMSP,OB,DL and HL,the targets were corrected to gene names via the Uniprot database,the related targets and genes of bradyarrhythmia were obtained using GeneCards database.The intersection targets were screened after analyzing the targets of the disease and drug targets via Venny diagram,compound-target interaction diagram was built via Cytoscape 3.6.1 software;PPI network was constructed based on STRING database;GO enrichment analysis and KEGG pathway enrichment analysis of the intersection targets were carried out utilizing David database.Results:All 36 effective active ingredients of Mahuang Fuzi Xixin Decoction in treating the disease have been screened,and the key compounds are quercetin and kaempferol;there were 65 targets related to the treatment of the disease,and the main ones contained IL-6,TNF,VEGFA,CXCL8 and IL-1B,mainly involving the adrenergic receptor signaling pathway activated by adenylate cyclase,positive regulation of nitric oxide biosynthetic processes,norepinephrine-adrenergic vasoconstriction involved in the regulation of systemic arterial blood pressure,positive regulation of angiogenesis,which could treat the disease through participating in TNF signaling pathway,Toll-like receptor signaling pathway,calcium signaling pathway and cGMP-PKG signaling pathway.Conclusion:The study has been a multifaceted investigation of the potential mechanism of Mahuang Fuzi Xixin decoction in the treatment of bradyarrhythmia,it is believed that the decoction could treat the disease from multi-ingredient,multi-target and multi-way,and it could produce the powerful evidence for clinical treatment of the disease.
维普
万方数据
