Protective Effect of Kidney Tonifying and Detoxifying Formula on Pregnant Mice with Nuclear Radiation
Objective:To observe the protective effect of the kidney tonifying and detoxifying formula on the reproductive system damage of female pregnant mice caused by nuclear radiation.Methods:Thirty C57BL/6J female pregnant mice were randomly divided into a blank group,a radiation group,and a traditional Chinese medicine group,with ten mice in each group.The traditional Chinese medicine group was given kidney tonifying and detoxifying formula by gavage,13.5 g/(kg·d),while the other two groups were given 0.2 mL of physiological saline by gavage.Each group was drenched for 20 days.Mice of the radiation group and traditional Chinese medicine group received full body irradiation with a one-time dose of 4Gy 60Co γ-ray on the 11th day of gavage.To observe the general condition,body mass,placental index,the contents of progesterone and estradiol of mice on the day after radiation and on the 10th day of radiation.Results:The mice in the blank group showed good mental health,shiny hair,normal dietary intake,and normal bowel movement.Pregnant mice in the radiation group and traditional Chinese medicine group manifested restlessness,decreased food and water intake,and dry stool on the same day after radiation;on the 10th day after radiation,the mice in the radiation group appeared dull and lusterless fur,lethargy,lack of vitality,limited water and food intake,and dry stools;mice in the TCM group exhibited normal hair,basically normal dietary and water intake,and normal stools.On the day of radiation,there was no statistical difference in the body weight of pregnant mice among the groups(P＞0.05).On the 10th day after radiation,the body weight of the mice in the blank group increased(P＜0.01),while the body weight of the radiation group and the traditional Chinese medicine group decreased(P＜0.01).The traditional Chinese medicine group was higher than the radiation group(P＜0.05).Compared with the blank group,the placental index,the contents of progesterone and estradiol in both the radiation group and the traditional Chinese medicine group decreased(P＜0.01),while the traditional Chinese medicine group was higher than the radiation group(P＜0.05).Conclusion:The kidney tonifying and detoxifying formula can improve the general state of irradiated pregnant mice,promote the recovery of body weight,the levels of estrogen and progesterone,and placental index in irradiated pregnant mice.
radiation damagethe kidney tonifying and detoxifying formulaprotective effectmice
万方数据
维普
