Effect of Allicin on Renal Fibrosis in Type 2 Diabetic Rats Based on TGF-β1/Smads Signaling Pathway
Objective:To investigate the effects of allicin on renal fibrosis and transforming growth factor-β1(TGF-β1)/Smads signaling pathway in rats with type 2 diabetes mellitus(T2DM),and to explore the mechanism of allicin on renal fibrosis induced by T2DM.Methods:All 50 SD rats were randomized into normal control group,the model group,low(5 mg/kg),moderate(10 mg/kg)and high(20 mg/kg)doses groups of allicin with ten rats in each group.Normal control group accepted normal feeding,the remaining groups were established into T2DM rat models by adopting high glucose and high fat diet plus intraperitoneal injection of streptozotocin(STZ).After successfully modeling,different groups accepted intraperitoneal injection of the medicine,once each day,for four weeks.After four weeks of intervention,to detect the levels of fasting blood glucose(FBG)of the rats in different groups,and to weigh the body mass;biochemical analysis was used to detect the levels of 24h UP,BUN and SCr;HE staining was carried out to perform histopathological examination of the kidney;Masson staining was used for the examination of renal fibrosis and calculate CVF;immunohistochemistry(IHC)was utilized to measure the protein expressions of TGF-β1,p-Smad2,p-Smad3,CO1 Ⅰ and Col Ⅲ in renal tissue.Results:Compared with normal control group,the pathological changes including glomerular enlargement,mesangial matrix thickening,vacuolar degeneration of renal tubular epithelial cells and inflammatory cells infiltration had been significantly improved in the model group,the levels of FBG,24h UP,BUN,SCr,CVF,TGF-β1,p-Smad2,p-Smad3,CO1 Ⅰ and Col Ⅲ raised while the body mass reduced;compared with the model group,the levels of FBG lowered while the body mass increased in the moderate and high doses groups of allicin(P＜0.05);the levels of 24hUP and serum BUN,SCr lowered(P＜0.01),the pathological changes improved;compared with the model group,the renal fibrosis of the rats in low,moderate and high doses groups of allicin has been improved,CVF in renal tissue was apparently lowered(P＜0.01);compared with the model group,the protein expressions of TGF-β1,p-Smad2,p-Smad3,CO1 Ⅰand Col Ⅲ in renal tissue were significantly downregulated in moderate and high doses groups of allicin(P＜0.01).Conclusion:Allicin could inhibit renal fibrosis in T2DM rats,and its mechanism might be related to the modulation of TGF-β1/Smad signaling pathway by allicin and thus the inhibition of extracellular matrix production.
type 2 diabetes mellitusrenal fibrosiscollagenTGF-β1/Smad signaling pathwayallicin
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