Effects of the Environments Enriched by Different Time Windows on the Changes of Learning and Memory Ability in Alzheimer's Disease Mice
Objective:To investigate the effects of enriched environment training through different time windows on the change of learning and memory ability in Alzheimer's disease(AD)mice.Methods:Thirty APP/PS1 mice were randomly divided into the model group,the 6-month-old EE group,the 9-month-old EE group,and ten C57BL/6 mice were chosen as the negative control group.The 6-month-old EE group was separated into EE6 h and EE12 h groups,enriched with environmental intervention for 6h and 12 h per day,respectively,at six months of age.The 9-month-old EE group was allocated to EE6 h and EE12 h groups,enriched with environmental intervention for 6 h and 12 h daily at nine months of age.After 14 days,the Morris water maze experiment was conducted to detect spatial learning and memory ability,and HE staining and Nissen staining were used to observe the hippocampal pathology and neuron number.Results:The differences in escape latency time and between-group effects were statistically significant in 6-month-old and 9-month-old groups of mice(P＜0.05).The 12 h EE group at the same month of age entered the target quadrant more often,crossed the platform more often and stayed in the target quadrant longer than the model group(P＜0.05).The HE staining and Nissl staining cells in each group of EE have a relatively complete morphology and concentrated distribution.The number of normal neurons in the hippocampus was significantly higher in the EE group than in the model group(P＜0.05).Conclusion:EE can improve learning and memory ability and slow down hippocampal neuron apoptosis in AD mice,and the effect of prolonged enriched EE is obvious,early intervention of disease is the best start time.
Alzheimer's diseaselearning and memory abilityenriched environmenttime window
万方数据
维普
