Effects of Leigongteng Medicated Wax on Joint Inflammation and Bone Erosion in Mice Models with Collagen-induced Arthritis
Objective:To investigate therapeutic effects of Leigongteng(Tripterygium wilfordii Hook.f.)medicated wax on joint inflammation and bone erosion in mice models of collagen-induced arthritis(CIA mice).Methods:Twenty-four DBA/1J male mice were randomly divided into three groups of eight mice each,namely,the normal group,the CIA group,and the Leigongteng medicated wax CIA group.Except for the normal group,bovine type Ⅱ collagen-induced arthritis mouse model was used in the rest of the groups;another eight DBA/1J male mice were taken for acute skin irritation test.The Leigongteng medicated wax CIA group was treated with Leigongteng medicated wax at the beginning of the second modeling immunization,to observe the degree of arthritic swelling,synovial inflammation and local bone erosion in each group of mice.Results:Skin irritation experiments showed that ordinary wax therapy and Leigongteng medicated wax had no irritation to the skin of mice;during the modeling period,joint swelling gradually appeared in the CIA group and the Leigongteng medicated wax CIA group,and some mice showed insignificant increase or even a slight decrease in body mass,a slight hair loss near the tail root,a slightly darker fur,and a decrease in activity and diet,in which the decrease in body mass of the Leigongteng medicated wax CAI group was slightly smaller than that of the CIA group,but when compared with the normal group,the difference had no significant meaning(P＞0.05);the arthritis score of the Leigongteng medicated wax CIA group was significantly lower than that of the CIA group(P＜0.01);hematoxylin-eosin staining(HE staining)suggested that compared with the ankle joints of the CIA mice,the joint space of the Leigongteng medicated wax CIA group was slightly narrowed,the joint destruction was reduced compared with that of the CIA group,and the synovial membrane was not obviously proliferated.However,there was still a small amount of inflammatory cell infiltration around the joints.The histopathological scores of joints in the Leigongteng medicated wax CIA group were significantly lower than those in the CIA group(P＜0.0001).Conclusion:Leigongteng medicated wax can significantly reduce the proliferation of synovial tissue and inflammatory cell infiltration,alleviate the degree of swelling of the joints,reduce the destruction of bone,which is a simple,safe and effective treatment for rheumatoid arthritis.
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万方数据
维普
