The Effect of Pachymic Acid on the Proliferation,Migration and Invasion of Mouse Melanoma Cells and Study on the Mechanism
Objective:To study the effects of pachymic acid(PA)on the proliferation,migration and invasion of mouse melanoma B16 cells and its possible mechanisms.Methods:CCK8 assay and cell clone formation assay were used to detect the effects of different concentrations(20,40,80 μmol/L)of PA on the proliferation of B16 cells;cell scratch and Transwell assays were used to observe the effects of PA on the migra-tion and invasive ability of B16 cells;and Western Blot and immunofluorescence assays to detect the effects of PA on the expression of epithelial-mesenchymal transition-related protein of B16 cells.Results:PA could obviously inhibit the proliferation of B16 cells in a concentration-dependent manner(P＜0.01);PA could remarkably inhibit the migration and invasion ability of B16 cells after 24 h of intervention(P＜0.01);40 μmol/L PA up-regulated the expressions of E-cadherin protein(P＜0.05)and down-regulated the expression of Vimentin protein of B16 cells(P＜0.05);immunofluorescence assay showed that 40 μmol/L PA apparently decreased the expression of Vimentin protein in B16 cells.Conclusion:PA can inhibit the proliferation,migration and invasion of B16 cells,and its mechanism may be related to the up-regulation of E-cadherin protein expression and the reduction of Vimentin protein expression in B16 cells,thus inhibiting the epithelial-mesenchymal transition of B16 cells.
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万方数据
维普
