Study on the Mechanism of Action of Mahuang-Guizhi Drug Combinations for the Treatment of Allergic Rhinitis based on Network Pharmacology and Molecular Docking
Objective:To study the mechanism of action of Mahuang(Ephedrae herba)-Guizhi(Cinnamomi ramulus)in the treatment of allergic rhinitis(AR)based on network pharmacology and molecular docking.Methods:The TCMSP and Unitprot databases were applied to screen the active ingredients and targets of Mahuang-Guizhi,and the OMIM,GeneCards,DrugBank,and TTD databases were used to obtain the targets of AR,and the intersection of these targets was used to obtain the targets of the drug pairs for the treatment of AR and to draw the Venny diagram;Cytoscape 3.7.2 software was used to construct the"drug-component-target-disease"network;STRING database and Cytoscape 3.7.2 software were applied to construct the protein-protein interaction network and perform topology analysis;Metascape database was utilized to perform KEGG enrichment and GO enrich-ment analysis;Pubchem database and chem3d software were used for molecular docking verification of key components and targets.Results:All 27 active ingredients and 109 targets acting on AR were obtained for the drug pair;the key active ingredients were mainly quercetin,β-sitosterol,kaempferol,lignocerol,etc.;the key targets were mainly TNF,RMLA,IL-6,AKT1,etc.;the therapeutic effects were mainly exerted through the signaling pathways such as PI3K-Akt,TNF,AGE-RAGE,IL-17,etc.;molecular docking results showed that the compo-nents and targets had good binding.Conclusion:The study elucidates the multi-ingredient,multi-target and multi-pathway mechanism of action of Mahuang-Guizhi in the treatment of AR,which may provide the direction and basis for further in-depth research.
NETL
NSTL
万方数据
维普
