Identification and Study of Tufuling and Red Woody Tufuling
Objective:To analyze the quality difference between Tufuling(smilacis glabrae rhizoma)and red woody Tufuling by comparing the character,microscopic identification,TLC,infrared chromatography and HPLC fingerprint.Methods:The character identification,cross section identification,water test identification,powder microscopic identification,TLC,infrared chromatography of two kinds of the herbs were observed,and HPLC was adopted to detect the contents of astilbin.Results:The property and microscopic identification of Tufuling and red woody Tufuling were noticeably different;the water test identification showed that the aqueous solution of Tufuling was always transparent,while the aqueous solution of red woody Tufuling was always turbid and opaque;the differences existed in TLC,infrared chromatography and HPLC fingerprint of both drugs.Conclusion:Tufuling section had a darker colour,fewer starch grains,slightly lower viscosity of water infusion and higher content of astilbin,which were significantly different from each other and were useful as references for the quality evaluation of Tufuling.