Preventive Effect of Aqueous Extract of Hongmao Wujia Leaves on Focal Cerebral Ischemic Injury in Rats
Objective:To study the protective effect of aqueous extract of Hongmao Wujia leaves(acanthopanax giraldii leaves extract,AGLE)on focal cerebral ischemic injury in rats.Methods:All 138 male SD rats were randomly divided into sham operation group,the model group,ginkgo biloba group(0.04 g/kg),and high,medium,and low dose groups(20,10,and 5 g/kg)of AGLE,23 ones in each group and focal cerebral ischemia model was prepared using the middle cerebral artery occlusion(MCAO)of rats by the wire-insertion method;the extent of cerebral infarction was determined by TTC staining;the neurological deficit scores of the rats were observed by the Longa method;and the pathological changes in the cerebral cortex on the ischemic side were observed under light microscope;the nitric oxide synthase(NOS)activity and nitric oxide(NO)content of brain tissue were measured by spectrophotometry and nitrate reductase method,respectively.Results:Behavioral index scores,extent of cerebral infarction,NOS activity and NO content were higher in the model group than in the sham-operated group(P<0.01);the cerebral cortex on the ischemic side showed pathological changes such as neuronal structural ambiguity,neuronal cell swelling,and nuclear consolidation,lysis,rupture or disappearance.Behavioral index scores,extent of cerebral infarction,NOS activity and NO content were all reduced in each dose group of AGLE compared with the model group(P<0.01).Compared with ginkgo biloba group,behavioral index scores were improved more obviously in low dose group of AGLE(P<0.01).The difference had no statistical meaning in the comparisons of extent of cerebral infarction,NOS activity and NO content between different doses groups of AGLE(P>0.05).Conclusion:AGLE has a preventive effect on focal cerebral ischemic injury in rats,and its mechanism of action may be related to the reduction of NOS activity and NO content in brain tissue.
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