Effects of Huangqin and Ginger on Gastrointestinal Function in Rat Models with Dampness-heat based on"Mutual Inhibition"
Objective:To compare the effects of Huangqin(Scutellariae radix)and ginger on the gastrointestinal function of rats modeled with dampness-heat before and after compatibility,and to investigate the connotations of"mutual inhibition"between the two drugs.Methods:Fifty SD rats were randomly divided into the normal group,the model group,Huangqin group,Huangqin-ginger group with different ratios(1:1,2:1),ten rats in each group.Rat models with gastrointestinal damp-heat were established using composite factors.The corresponding drugs were given by gavage once a day for seven consecutive days after modeling;after sampling,HE was applied to observe the histopathological and morphological changes in the stomach and colon of rats in each group under the light microscope;to calculate the residual rate in the stomach;nitrate reductase method was used to detect the levels of serum NO;ELISA was adopted to detect the expressions of TNF-α and IL-10 in the serum in different groups;ELISA was used to measure the contents of SP,VIP,SS,GAS in the stomach and colon.Results:After the intervention,compared with the model group,the residual rate of the stomach decreased in Huangqin group,and Huangqin-ginger at the ratio of 1:1(P<0.05),Huangqin decoction and Huangqin-ginger decoction at the ratio of 1:1 down-regulated the levels of NO and TNF-α,and up-regulated the levels of IL-10 in rats(all P<0.05),Huangqin-ginger decoction at the ratio of 1:1 decreased the contents of VIP and SS in the stomach and colon of rats with dampness-heat and elevated the contents of SP and GAS(all P<0.05).Compared with Huangqin-ginger decoction and Huangqin-ginger decoction at the ratio of 1:1,Huangqin-ginger decoction at the ratio of 2:1 down-regulated the levels of serum NO,the levels of VIP and SP in stomach and colon,up-regulated the levels of CAS in colon insignificantly(P>0.05).Conclusion:Huangqin decoction and Huangqin-ginger decoction at the ratio of 1:1 can improve the state of gastrointestinal function in rats with dampness-heat,mainly through regulating the levels of gastrointestinal hormones and inflammatory factors.When Huangqin was combined with ginger at the ratio of 2:1,the two drugs inhibited each other,and the effect of clearing heat and drying dampness was weakened.
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