归龙丸对高糖诱导的RSC96神经膜细胞分泌TNF-α、IL-10的影响
Effects of Guilong Pills on TNF-α and IL-10 Secretion of RSC96 Schwann Cells Induced by High Glucose
王文英 1李宝玲 1黄宏羽 1何万辉1
作者信息
- 1. 广州医科大学附属中医医院,广东 广州 510130
- 折叠
摘要
目的:评估归龙丸对高糖诱导的大鼠神经膜细胞(rats schwann cell,RSC96)分泌肿瘤坏死因子α(tumor necrosis factor α,TNF-α)、血清白细胞介素10(interleukin-10,IL-10)的影响.方法:将RSC96神经膜细胞复苏及培养,在含17 mmol/L的葡萄糖培养基中培养48 h.分为S1组(维生素D3+等渗乙醇溶液)、S2组(维生素D3+等渗乙醇溶液+归龙丸高剂量)、S3组(维生素D3+等渗乙醇溶液组+归龙丸中剂量)、S4组(维生素D3+等渗乙醇溶液组+归龙丸低剂量)、S5组(维生素D3+CYP24A1抑制剂genistein的乙醇溶液)等5组,S1～S4组中分别加入维生素D3及与其等浓度的乙醇,S5组加入维生素D3及CYP24A1抑制剂genistein的乙醇溶液,作用48 h.48 h后应用酶联免疫吸附试验法检测各组细胞上清液中TNF-α、IL-10的水平,并通过相应的基因引物检测RSC96细胞内CYP24A1m表达水平.结果:经归龙丸和CYP24A1抑制剂组处理后的神经膜细胞,其TNF-α的水平下降,IL-10水平上升,CYP24A1m表达水平下调.结论:归龙丸可能通过抑制CYP24A1蛋白表达,间接调节维生素D3使神经膜细胞TNF-α分泌减少、IL-10分泌增加,从而对高糖诱导的RSC96神经膜细胞起到保护作用.
Abstract
Objective:To evaluate the effects of Guilong pills on the secretion of TNF-α and IL-10 in RSC96 Schwann cells induced by high glucose.Methods:After being resuscitated and cultured in a medium containing 17 mmol/L glucose for 48 h,RSC96 Schwann cells were divided into five groups:the S1 group(Vitamin D3+isotonic ethanol solution group),the S2 group(Vitamin D3+isotonic ethanol solution+high dose group of Guilong pills),the S3 group(Vitamin D3+isotonic ethanol solution+moderate dose group of Guilong pills),the S4 group(Vitamin D3+isotonic ethanol solution+low dose group of Guilong pills)and the S5 group(Vitamin D3+ethanol solution group of CYP24A1 inhibitor genistein),Vitamin D3 and the same concentration of ethanol solution were added respectively into the S1-S4 groups,ethanol solution of vitamin D3 and CYP24A1 inhibitor genistein was added to group S5 and acted for 48 hours.ELISA method was applied to detect the levels of TNF-α and IL-10 in the cell supernatant of each group in 48 hours,and the corresponding gene primer was used to measure the levels of CYP24A1m in RSC96 cells.Results:The levels of TNF-α decreased while the levels of IL-10 increased,the expressions of CYP24A1 downregulated in RSC96 Schwann cells processed with Guilong pills and CYP24A1m group.Conclusion:Guilong pills could reduce the secretion of TNF-α and increase the levels of IL-10 possibly by inhibiting the expressions of CYP24A1 protein,and indirectly regulating the levels of Vitamin D3,thereby protecting RSC96 Schwann cells induced by high glucose.
关键词
归龙丸/高糖/RSC96神经膜细胞/肿瘤坏死因子α/血清白细胞介素10Key words
Guilong pills/high glucose/RSC96 Schwann cells/TNF-α/IL-10引用本文复制引用
基金项目
广东省自然科学基金面上项目(2021A1515012547)
第四批全国中医优秀人才研修项目(国中医药人教发[2017]24号)
广州市中医药科技项目(20172A010012)
出版年
2024
国家科技期刊平台
NSTL
万方数据