Objective:To investigate the hepatotoxicity of compound Chuancaowu(Aconiti kusnezoffii Radix)mixture on mice,and the analgesic and anti-inflammatory effects of the medicine.Methods:All 48 male ICR mice were randomized into the normal group,the model group,low(0.33 g/mL),moderate(0.66 g/mL)and high(1.32 g/mL)doses groups of compound Chuancaowu mixture,and normal+high doses group of compound Chuancaowu mixture with eight mice in each group.The mice remained unhandled,other groups were established into the models by subcutaneous injection of complete freund's adjuvant(CFA)into the plantar surface of mice,all the doses groups of compound Chuancaowu mixture were drenched with the corresponding doses of compound Chuancaowu mixture,the normal group and the model group accepted intragastric administration of double distilled water.To detect mechanical stimulation pain threshold and thermal radiation pain threshold of the mice in different groups at different time points;after seven days of intervention,to observe histopathological changes of liver and kidney in the mice of different groups;ELISA method was used to measure the contents of ALT,AST,DB,TB,CRE,UA and UREA of the mice in different groups and the levels of IL-1β in the foot.Results:Compared with the model group,the mixture could lift mechanical stimulation pain threshold and latency time of thermal radiation in mice induced by CFA in the dose-dependent manner(P<0.05);the hepatocytes were arranged radially with no obvious abnormalities,the hepatic lobule structure was clear and the overall structure of the renal tubules was intact in the normal group and normal+high doses group of compound Chuancaowu mixture;the difference had no stastical meaning in the contents of ALT,AST,DB,TB,CRE,UA and UREA between different groups(P>0.05);compared with the model group,the contents of IL-1β were reduced in the feet of the mice in moderate and high dose groups of compound Chuancaowu mixture(P<0.05).Conclusion:Compound Chuancaowu mixture has the analgesic and anti-inflammatory effects on CFA-induced chronic inflammatory pain in mice without tolerance and obvious hepatotoxicity.
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