附子丁香散加味穴位贴敷对便秘大鼠结肠组织PGP9.5及BDNF蛋白表达的影响
Influence of Acupoint Application of Modified Fuzi Dingxiang Powder on the Expressions of PGP9.5 and BDNF Protein in Colon Tissue of Constipation Rats
赵芮 1韩玮玮 2王本军 2祝颂3
作者信息
- 1. 山东中医药大学,山东 济南 250014;山东中医药大学附属医院肛肠科,山东 济南 250014
- 2. 山东中医药大学,山东 济南 250014
- 3. 山东中医药大学附属医院肛肠科,山东 济南 250014
- 折叠
摘要
目的:探讨附子丁香散加味穴位贴敷对阳虚型慢传输型便秘(slow transit constipation,STC)大鼠结肠组织中蛋白基因产物 9.5(protein gene product 9.5,PGP9.5)及脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)蛋白质表达的影响.方法:将60只Wistar大鼠随机分为对照组、造模组、穴贴组各20只.造模组和穴贴组每3.0 mg/kg体质量大鼠灌胃咯哌丁胺悬液,持续21天,同时每日予氢化可的松注射液25 mg/(kg·d)后肢肌肉注射进行造模;对照组灌胃等体积蒸馏水.造模成功后,造模组大鼠清水灌胃21天,每日1次;穴贴组在大鼠神阙及双天枢穴敷贴附子丁香散加味药饼,每日1次,每次6 h,连贴6天后暂停1天,共进行3个循环.末次灌胃后24 h处死大鼠,通过HE染色、免疫组织化学方法观察大鼠结肠形态和病理变化,并检测结肠组织中PGP9.5及BDNF蛋白表达水平.结果:组织病理学显示对照组大鼠近端结肠层次清晰,腺体齐整,肌间神经丛内见正常神经节,神经元细胞阳性表现为分布均匀的棕色染色;造模组大鼠肠黏膜水肿,肠壁变薄,炎症细胞浸润,肠壁肌纤维大部分断裂,空泡样变性,神经元细胞大量减少,细胞阳性表达染色降低;穴贴组大鼠肠壁及腺体有所恢复,结肠肌间神经丛神经元细胞与造模组相比,空泡变性多数已恢复,炎症细胞浸润显著减少.治疗后穴贴组大鼠结肠组织PGP9.5和BDNF表达均高于造模组(P＜0.05).结论:附子丁香散加味穴位贴敷可能通过提高PGP9.5及BDNF蛋白在结肠组织中的表达来治疗STC.
Abstract
Objective:To discuss the influence of acupoint application of modified Fuzi Dingxiang powder on the expressions of PGP9.5 and BDNF in colon tissue of rats with Yang deficiency pattern of slow transit constipation(STC).Methods:Sixty Wistar rats were randomized into the control group and the modelling group and the acupoint application group with 20 rats in each group.The modelling group and the acupoint application group were drenched with loperamide suspension per 3.0 mg/kg of the body mass,continuously for 21 days,meanwhile they accepted muscular injection of hydrocortisone injection into the hind limbs,25 mg/(kg·d),to establish the models;the control group were gavaged with an equal volume of distilled water.After successfully modeling,the rats in the modeling group were drenched with clean water,once each day;the acupoint application group accepted external application of modified Fuzi Dingxiang powder at Shenque(CV8,RN8)and Tianshu(ST25)bilaterally of the rats,once each day,six hours each time,applying for six days and resting for one day,three cycles altogether.The rats were sacrificed in 24 hours after the final gavage,HE staining and immunohis-tochemical methods were applied to observe the morphology of the colon and pathological changes of the rats,and to detect the expressions of PGP9.5 and BDNF in colon tissue.Results:Histopathology showed that the proximal colon of control rats was well layered with flush glands,normal ganglia were seen within the intermuscular plexus,and neuronal cells positively showed evenly distributed brown staining;rats in the modelling group had intestinal mucosa edema,thinning of the intestinal wall,infiltration of inflammatory cells,most of the muscle fibres of the intestinal wall were broken,with vacuolike degeneration,a large reduction in neuronal cells and a decrease in positively expressed cellular staining;the intestinal wall and glands of the acupoint application group have recovered,the vacuolation of colon interosseous plexus neuronal cells mostly recovered,and the infiltration of inflammatory cells decreased significantly when the acupoint application group was compared with the modeling group.After the treatment,the expressions of PGP9.5 and BDNF in colon tissue of acupoint applicaion group were higher than these of the modeling group(P<0.05).Conclusion:Acupoint application of modified Fuzi Dingxiang powder could treat STC possibly,through raising the expressions of PGP9.5 and BDNF protein in colon tissue.
关键词
便秘,阳虚/慢传输型/基因蛋白产物9.5/脑源性神经营养因子/穴位贴敷/大鼠Key words
constipation,Yang deficiency/slow transit/protein gene product 9.5/brain-derived neurotrophic factor/acupoint application/rats引用本文复制引用
基金项目
山东省自然科学基金(ZR2015HL109)
山东省中医药科技发展计划(2017-059)
山东省中医药科技发展计划(2019-0140)
出版年
2024
国家科技期刊平台
NSTL
万方数据