Objective:To provide the reference for the identification of Dahua Hongjingtian[Rhodiola crenulata(Hook.f.et Thoms.)H.Ohba]by analyzing the base sequences of DNA bar codes of Dahua Hongjingtian and Changbian Hongjingtian[Rhodiola fastigiata(HK.f.et Thoms.)S.H.Fu]and constructing the phylogenetic tree.Methods:The fragments of internal transcribed spacer(ITS)were amplified by PCR and sequenced in two directions to obtain their sequence information after extracting the genomic DNA of three samples;MEGA 7.0 was used to compare the ITS sequences of all the samples and GenBank,a total of 21 entries,calculate the intraspecific interspecific genetic distances,and construct Neighbor-Joining(NJ)phylogenetic trees.Results:Numbers A1-A14 Dahua Hongjingtian clustered into one unit,and numbers K1-K7 Changbian Hongjingtian clustered into one unit;meanwhile,the variation of the ITS sequence information was obviously correlated with the geographic areas and environments.Conclusion:ITS sequence as DNA bar code can accurately and quickly identify Dahua Hongjingtian,which could provide an important basis for its germplasm resource identification and species identification.
Dahua HongjingtianChangbian HongjingtianDNA bar codeITSidentification
NETL
NSTL
万方数据
