Study on HPLC Specific Chromatograms and Multi-component Content Analysis of Danggui Formula Granules and Decoction Pieces
Objective:To establish high performance liquid chromatography(HPLC)characteristic chroma-tograms of Danggui(Angelica sinensis)slices and its formula granules,and to conduct correlation evaluation and content analysis of the four components.Methods:The fingerprints of 18 batches of samples were plotted using the"similarity evaluation system for chromatographic fingerprint of TCM(version 2012)"to investigate the similarity.Cluster analysis was performed with SPSS 22.0 software.The correlation evaluation and the content analysis of four components were performed on Danggui and the formula granules of two manufacturers(Manu-facturer A and Manufacturer B).Results:There are 18 common peaks in the HPLC characteristic chromatograms of Danggui decoction pieces and the formula granules of the two manufacturers,with similarities ranging from 0.698 to 1.000;four characteristic peaks of chlorogenic acid,ferulic acid,ligustilide I and ligustilide were identified,and the linear equations are Y=8.5183X+40.389(R2=0.9995),Y=19.951X+4.7477(R2=0.9994),Y=52.466X+17.971(R2=0.9994),Y=5.03X+6.68(R2=0.9993),the average sample recovery rates were 99.60%,99.09%,98.13%,and 98.09%respectively.RSDs of the repeatability,precision,and stability tests were all less than 3%;the cluster analysis results showed that when the distance was 2,18 batches of the samples can be clustered into two categories,of which S1 to S6 and S7 to S18 are each clustered into one category,which is consistent with the similarity evaluation results.Compared with the decoction pieces,peak No.4 is added to the formula particles,and peak No.18 has a large difference.Conclusion:The HPLC fingerprints of Danggui decoction pieces and formula granules,between formula granules show high similarity,but there are still differences,which could provide a reference for the quality control and clinical application of formula granules.
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