Influence of Zuogui Pills on Peripheral Blood Cells and the Spleen Indexes in Rats with 60 Coγ-rays Injury
Objective:To discuss the influence of Zuogui pills on the levels of peripheral blood cells and sex hormone and IL-1β,the number of ovarian follicles as well as spleen indexes in SD rats suffering 60Coγ-rays injury.Methods:A total of 50 female SPF grade SD rats at eight weeks of age were selected,ten of them were in the normal group,not irradiated and routinely reared;the rest 40 were divided into radiation model group,estradiol valerate tablets group,high and low dosage groups of Zuogui pills after irradiated with 60Coγ-rays(6.0Gy,LD40)for 24 h.Estradiol valerate tablets were administered to estradiol valerate tablets 0.09 mg;high and low dosage groups of Zuogui pills were given Zuogui pills at the concentrations of 4.725 g crude drug and 0.945 g crude drug;radiation model group was drenched with physiological saline 2 mL,once each day,and intervened for 21 days.To detect the changes of peripheral blood cells at different time intervals;ELISA was used to detect the expressions of FSH,E2,LH and IL-1β in peripheral blood,to measure the number of ovarian follicles and spleen indexes.Results:After being irridated,the number of peripheral blood cells was decreased,and the reduction of white blood cells was most pronounced(P<0.05),the contents of hemoglobin were increased(P<0.05),the level of E2 was lowered,the number of mature follicles was reduced,spleen index was lowered,and the expressions of IL-1β were lifted;after the intervention of Zuogui pills,the levels of E2,the number of mature follicles and spleen indexes were increased,the levels of IL-1β,FSH and LH were lowered,especially the effects were more pronounced in the high-dose group of Zuogui pills(P<0.05).Conclusion:Zuogui pills could promote the restoration of ovarian function in rats with radiation damage possibly through promoting bone marrow hematopoiesis and immune function,which reflects the theory of"kidney governing the blood vessels".
NETL
NSTL
万方数据
