Effects of Microbial Fertilizer on Physiological Characteristics of Malus sieversii under Alkali Stress
[Objective]The effects of microbial fertilizer with Bacillus licheniformis as the main functional bacteria on the physiological characteristics of Malus sieversii seedlings under alkali stress were determined by measuring the related physio-logical indexes of Malus sieversii seedlings under microbial agents and alkali treatment,so as to provide theoretical basis for improving the stress resistance of Malus sieversii seedlings.[Methods]The potted Malus sieversii seedlings were used as ex-perimental materials,and the potted soil was irrigated with 1∶1 000 compound microbial bacteria solution.After one month,the potted soil was treated with NaHCO3∶Na2CO3 as 1∶1 alkali solution.The control group was treated with clear water to ir-rigate the potted seedlings without alkali solution.The control group(CK),alkali stress group and alkali+bacterial fertilizer group were set up.The pH and conductivity of the soil under different treatments and the photosynthetic pigments,photosyn-thetic parameters,osmotic adjustment substances and antioxidant enzyme activities of Malus sieversii seedlings were measured and analyzed.[Results]The findings demonstrated that the application of microbial fertilizer effectively mitigated the detrimen-tal effects of alkali stress on Malus sieversii seedlings.This was achieved through alterations in soil pH and EC,reduction in membrane lipid peroxidation,enhancement of chlorophyll content,elevation of carotenoid to total chlorophyll ratio,mainte-nance of stomatal conductance and intercellular CO2 concentration,as well as regulation of osmoregulatory substance levels.[Conclusion]The application of microbial fertilizer can mitigate soil salinity and modulate the physiological response of Malus sieversii seedlings,thereby enhancing their adaptability to alkali stress.
Malus sieversiimicrobial fertilizeralkaline resistancephysiological character