Study on Ropivacaine inhibits Cervical Cancer Cells Via the JAK-PI3K-STAT Pathway
OBJECTIVE To investigate the effects of ropivacaine on cervical cancer Helacells and provide experimental evidence for the selection of anesthetic agents in cervical cancer surgery.METHODS Different concentrations of ropivacaine were applied to cervical cancer Helacells.Cell proliferation capacity was assessed using the MTT assay,apoptosis was examined by flow cytometry,and changes in cell invasiveness were determined using the Transwell chamber assay.A xenograft model of human cervical cancer Hela cells in nude mice was established to observe the impact of ropivacaine treatment on tumor cell growth.The phosphorylation level of STAT3 and the expression levels of JAK,PI3K,and AKT proteins were evaluated using Western blot analysis,along with the assessment of STAT3 phosphorylation levels after inhibition and ropivacaine treatment.RESULTS Ropivacaine significantly inhibited the growth and migration capacity of Hela cells,promoted apoptosis,and suppressed STAT3 phosphorylation and the JAK-PI3K-AKT pathway.Animal experimental results demonstrated that ropivacaine could inhibit the growth of cervical cancer cells.CONCLUSION Ropivacaine exerts an inhibitory effect on cervical cancer cells by suppressing the JAK-PI3K-AKT pathway.
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