Network pharmacologyapproach for the mechanism of Huabeixiaocuo Decoction in Acne
OBJECTIVE To explore the molecular mechanism of Huabeixiaocuo decoction in the treatment of acne based on network pharmacology and molecular docking technology.METHODS The main active components of Huabeixiaocuodecoction were screened by TCMSP and ETCMdatabase and the targets were predicted by SwissTargetPrediction website.At the same time,the related targets of ance were screened from Gene Card database,Disgent database and TTD database.The protein-protein interaction network was constructed using String website and Cytoscape 3.9.1 software after mapping the component target and disease target.Molecular docking technology was used to match potential active components with key targets.GO and KEGG were analyzed by Metascape website.Cytoscape3.9.1 software was used to construct herb-component-target-pathway network.RESULTS 207 active ingredients and 1 722 potential targets were screened from Huabeixiaocu odecoction,among which 70 core targets and 20 pathways were closely related tothe treatment of acne.Molecular docking results showed that the active ingredients and their key targets had good binding activity.CONCLUSION Huabeixiaocuo decoction has biological support in treating acne.Different proteins have synergistic effects,in which SRC,MAPK1,STAT3,RELA,RXRA,EP300,NFKB1 and NFKBIAetc.play a key role.Biological processes are mainly involved in hormone synthesis,endocrine regulation,angiogenesis,cell signal transduction,inflammation etc.These targets are mainly concentrated in multiple signaling pathways such as MAPK and Rap 1 signal pathway,which play key roles of anti-inflammatory,anti-skin keratosis,regulation of cell adhesion,repair of skin barrier and so on.
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