Molecular Mechanism of Allicin in the Treatment of Acute Cystitis Inflammation Induced by Escherichia Coli
OBJECTIVE To delve into the therapeutic role of allicin in addressing Escherichia coli-triggered acute cystitis and the potential underlying mechanisms.METHODS An acute cystitis model was established in rats,and treated with allicin administration.The pathological changes of rat bladder tissues were observed by HE staining to observe allicin's therapeutic effect.The bladder tissue transcriptome sequencing was conducted across distinct rat groups.In tandem with GEO data analysis,downstream targets influenced by allicin were discerned.Potential molecular targets were screened through GO,KEGGenrichment analysis,and protein-protein interaction analysis(PPI).The experimental ofimmunohistochemistry on rat bladder tissue sections was valiated.RESULTS The results depicted allicin diminished structural disarray within rat bladder tissue sections and reduced immune cell infiltration,showing substantial therapeutic efficacy against acute cystitis.Transcriptome sequencing of rat bladder tissues revealed allicin's significant suppression of 115 upregulated proteins and induction of 24 downregulated proteins in acute cystitis,amalgamated with C57 mouse bladder inflammation sequencing data from GEO,37 commonly upregulated proteins and 1 downregulated protein were identified.Functional enrichment analysis showed that the dominance of immune-related pathways,encompassed immune response regulation,T cell receptor signaling,Th 17 cell differentiation,Th l and Th2 cell differentiation,cytokine-immune factor receptor interaction and more.Further dissection via PPI identified immune cell-associated factors such as CD74,CD96,ICOS,PTPN22,PDCD1,and CXCR6.Combined with their sequencing abundance of in rats,CD74 with the most significant difference was finally selected.Empirical findings furthervalidatedthat allicin curbed urinary epithelial CD74 expression,mitigated macrophage infiltration dispersion in E.coli-induced acute cystitis,ameliorated inflammatory response.CONCLUSION This study unearths allicin's potential in E.coli-induced acute cystitis treatment,providing a method for exploring the therapeutic potential of natural antimicrobial monomers like allicin in managing acute cystitis.
万方数据
维普
