Contents Differentiation of Ginsenoside Components in Panax Ginseng,Red Ginseng,American Ginseng by UPLC-MS/MS
OBJECTIVE To detect the contents of ten ginsenoside compounds in Panax Ginseng,red ginseng,American ginseng and their different parts,and to analyze and evaluate their quality.METHODS The chromatographic column was Waters ACQUITY HPLC BEH C18 Column(2.1 mm× 100 mm,1.7 μm).The mobile phase was composed of 0.1％formic acid and acetonitrile-tetrahydrofuran(95∶5)in gradient elution at a flow rate of 0.3 mL·min-1.A triple quadrupole mass spectrometer operating in positive electrospray ionization mode with multiple reaction monitoring was used.RESULTS The linear relationships of ten ginsenoside compounds were good in corresponding ranges with correlation coefficients(r)all above 0.995.The average recoveries of ten ginsenoside compounds were 87.7％-111.2％with the RSDs within 1.9％-4.8％.The limits of detections(LODS)for ten ginsenoside compounds ranged from 0.005-0.01 μg·mL-1.The limits of quantitation(LOQS)ranged from 0.017-0.03 μg·mL-1.CONCLUSION The method not only is convenient,rapid,sensitive and accurate,but also can be used for quantitative determination of ten ginsenoside compounds in Panax Ginseng,red ginseng,American ginseng and their different parts,Simultaneously.The total amount of ginsenoside contained in non medicinal parts such as fibrous roots and reed heads of ginseng,red ginseng and American ginseng is high,which should be fully utilized to avoid waste of traditional Chinese medicine resources.
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