OBJECTIVE The identification and fingerprint are established to characterize the quality difference of Lycii Cortex from different sources,based on DNA barcoding and UPLC fingerprint technology.METHODS The identification was performed by ITS2 sequence based on DNA barcoding technology and the UPLC fingerprint was established to distinguish the differences of materials by combining cluster analysis,principal component analysis and discriminant analysis.RESULTS 17 batches of the materials were identified as 10 batches of Lycium chinense Mill.,4 batches of Lycium barbarum L.,and 3 batches of Lycium ruthenicum Murray.Ten common peaks were obtained from the fingerprint.Through principal component analysis and discriminant analysis,S1-S10,S11-S14 and S15-S17 were respectively classified as one class,which basically realized the distinction of different original samples.At the same time,the main differential compounds identified by different sources were obtained,which were peak 1',peak 8,peak 2,peak4,peak 1 and peak 3.And The different parts could be distinguished by peak 3.CONCLUSION The difference components of different original samplesare phenolic amides,alkaloids and Lyciuminetc.This study laid a foundation for the quality evaluation of Lycii Cortex from different sources by comprehensive evaluation of DNA barcoding and chemical identification models.
关键词
地骨皮/不同部位/DNA条形码/UPLC指纹图谱/化学识别模式
Key words
Lycii Cortex/different parts/DNA barcoding/UPLC fingerprint/chemical identification model
维普
万方数据