OBJECTIVE To determine the ABTS free radicals scavenging ability of Shengmai capsule by ultraviolet spectroscopy and thin-layer bioautography.METHODS The ABTS free radicals scavenging ability of medicinal herbs contained in Shengmai capsule was determined by ultraviolet spectroscopy and thin-layer bioautography.Those had strong antioxidant ability were selected for material analysis.RESULTS The results of ultraviolet spectroscopy showed that Shengmai capsule had the strong ABTS free radicals scavenging ability.Among the medicinal herbs contained in Shengmai capsule,Schisandra Chinensis had the strongest ABTS free radicals scavenging ability.Lignan components had the strong ABTS free radicals scavenging ability,in which,the ability of schisanhenol was strongest.Combined with the results of ultraviolet method,the ABTS free radical scavenging ability of Shengmai capsule was determined by thin-layer bioautography.The results of thin-layer bioautography were basically consistent to those of ultraviolet spectroscopy.CONCLUSION Shengmai capsule has strong scavenging ability of ABTS free radical.Using schisandra chinensis as a reference medicinal material and Schisandra phenols as indicator components,thin-layer bioautography can be usued for determining the scavenging activity of ABTS free radical in Shengmai capsules.
维普
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