Study on Rapamycin Facilitating Fear Memory Extinction in A PTSD Mouse Model
OBJECTIVE To investigate the effect of rapamycin on fear memory extinctionin PTSD model mice by promoting autophagy.METHODS Amouse model of post-traumatic stress disorder(PTSD)was established through conditioned fear extinction training.Subsequently,western blotting was employed to assess levels of microtubule-associated protein 1 light chain 3(LC3)and Beclin-1 and LC3 expression was assessed through immunofluorescence staining.Rapamycin was administered into the hippocampus of mice via stereotaxic brain injection.The impact of rapamycin on fear memory extinction was assessed through conditioned fear memory extinction training experiments.RESULTS Compared with the untrained group,the expressions of autophagy-related proteins Beclin-1 and LC3-Ⅱ significantly increased following fear memory extinction training(P＜0.01).Additionally,after treatment with rapamycin(10 μmol·L-1)on HT22 cells,rapamycin enhanced the immunostaining puncta of autophagy protein LC3 and promoted fear memory extinction(P＜0.05).CONCLUSION This study found that rapamycin can promote fear memory extinction and reduce fear memory levels,possibly through an increase in autophagy levels.These findings provide a theoretical basis for drug development for PTSD.
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