Discuss of Benzoic Acid and Sorbic Acid in Children's Vitamin and Mineral Oral Solution
OB JECTIVE To establish an HPLC method for the determination of benzoic acid and sorbic acid in children's vitamin and mineral oral solutions.METHODS The HPLC method utilized a diode array detector and C18 column.The mobile phase was mixture of methanol and 0.01 mol·L-1 ammonium acetate solution(with the pH adjusted to 8.0 using ammonia solution,5∶95).The flow rate was 1.0mL·min-1 and the detection wavelength was 230 nm.The injection volume was 10 μL.RESULTS Benzoic acid and sorbic acid showed a good linear relationship with the peak area in the range of 0.996 0 to 79.680 3 μg·mL-1 and 1.040 9 to 83.269 4 μg·mL-1,respectively.The average recoveries were 97.86％(n=9)and 99.74％(n=9).CONCLUSION This method is accurate,reliable,and reproducible,and can be used for the determination of benzoic acid and sorbic acid in children's vitamin and mineral oral solutions.
HPLCchildren's vitamin and mineral oral solutionbenzoic acidsorbic acid
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