Purification of Flavonoids from Leaves of Aquilaria sinensis by Ultrafiltration Membrane
OBJECTIVE To purify the extract of Aquilaria sinensis leaves by ultrafiltration membrane and determine the optimal purification process for flavonoids in the leaves.METHODS The flavonoid content in Aquilaria sinensis leaves was determined by the aluminum nitrate chromogenic method.The evaluation index was the effects of different dilution ratios,operation times,temperatures,and pressures on the retention rate of total flavonoids with a 1 × 104 Da organic membrane.The optimal process conditions were studied.RESULTS and CONCLUSION Using a 1 ×104 Da organic ultrafiltration membrane without dilution,an operation time of 30 min,an operation temperature of 40 ℃,and an operation pressure of 0.2 MPa,the ultrafiltration effect was better under the conditions.
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