OBJECTIVE To studya colorimetric sensor for the rapid detection of multiple mycotoxins in cosmetics and traditional Chinese medicine(TCM).METHODS Specific binding between target and aptamer and salt-induced interactions were used to control the aggregation of gold nanoparticles.When the targetwas present,the color change from red to purple-blue of the gold nanoparticle solution could be observed by naked eye,so as to carry out qualitative analysis.The quantitative analysis was performed using UV absorbance measurements.RESULTS Under the optimal conditions,the limit of detection(LOD)of oclatoxin A and aflatoxin B1 was as low as 0.001 8 ng·mL-1 and 0.018 ng·mL-1 respectively,and the recoveries were 89.51％-90.16％and 89.40％-97.77％,respectively.CONCLUSION The colorimetric sensor based on gold nanoparticle-aptamer is a simple,ultra-sensitive and low-cost detection method,which has a good prospect for the detection of various mycotoxins in food,drugs and cosmetics.
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