The tissue culture and rapid propagation of Dendrobium officinale
Objective To explore suitable culture mediums for the seed germination,seedling multiplication,rooting and growth of plantlet in Dendrobium officinale,in order to establish a tissue culture and rapid propagation system.Methods Seeds of Dendrobium officinale were used as the explants,and the effects of different mediums on seeds' sprouting,proliferation,rooting and seedling,such as hormone concentrations and natural additives were investigated.Results The most suitable culture medium for seed germination and proliferation were 1/2MS +0.2 mg/L NAA + 100 g/L potato +25 g/L sucrose and 1/2MS +0.2 mg/L NAA +100 g/L potato(banana) +25 g/L sucrose +0.5 g/L AC,respectively.The most suitable culture medium for rooting and seedling was 1/2MS +0.2 mg/L NAA + 100 g/L banana +25 g/L sucrose +0.5 g/L AC.Conclusion This research established a tissue culture and rapid propagation system,which could be used for the large scale production of plantlets.
Dendrobium officinaleseedtissue culture and rapid propagation
国家科技期刊平台
NSTL
万方数据
维普
