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香鳞毛蕨药材的质量标准研究

Study on quality standards of Dryopteris fragrans (L.) Schott

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目的 建立香鳞毛蕨药材质量标准,为控制其质量提供试验依据.方法 采用性状、显微鉴别法对香鳞毛蕨药材的性状、显微特征进行描述;参照2015年版《中国药典》四部通则相关方法,对该药材水分、总灰分、酸不溶性灰分、醇溶性浸出物进行测定;采用薄层色谱法,分别以绵马素BB(aspidin BB)和对照药材为对照,对该药材进行定性鉴别;采用紫外分光光度法,以绵马素BB为指标,建立了该药材中总间苯三酚质量分数的测定方法.结果 香鳞毛蕨药材的性状、显微、薄层鉴别方法均具有很好的专属性,6批样品的水分、总灰分酸、不溶性灰分、醇溶性浸出物平均值分别为9.45%、9.38%、1.58%、20.8%,总间苯三酚质量分数以绵马素BB计,为40.78 mg/g.结论 建立了香鳞毛蕨药材质量标准,所建立的质量标准可用于香鳞毛蕨的质量控制.
Objective To establish quality standards of D.fragrans,and provide scientific evidence for its quality control.Methods The microscopic identification was adopted to observe morphological and histological characters.The contents of water,total ash,acid-insoluble ash and alcohol-soluble extractive were analysed according to the methods of Chinese Pharmacopoeia (2015).Aspidin BB and reference herbs were used to identify materia medica of D.fragrans by TLC method.The total phloroglucinols contents were analysed by UV method with aspidin BB as index.Results The macroscopical identification,microscopic features and TLC methods were specific.The average contents of water,total ash,acid-insoluble ash,alcohol-soluble extract were 9.45%,9.38%,1.58% and 20.8%,respectively,and the content of total phloroglucinols was 40.78 mg/g.Conclusion The established quality standards were suitable for the quality control of D.fragrans.

D.fragransquality standardsaspidin BBtotal phloroglucinolsfast Blue B Salt

刘海燕、杜文钊、宋国强、陈文浩、袁丽鹏、沈志滨

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广东药学院中药学院,广东广州510006

香鳞毛蕨 质量标准 绵马素BB 总间苯三酚 固蓝B盐

广东省应用型科技研发专项

2015B020234009

2016

广东药科大学学报
广东药学院

广东药科大学学报

CSTPCD
影响因子:0.698
ISSN:1006-8783
年,卷(期):2016.32(1)
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